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| Heymann, Dominique; Ruiz-velasco, Carmen; Chesneau, Julie; Ratiskol, Jacqueline; Sinquin, Corinne; Colliec-jouault, Sylvia. |
| Osteosarcoma is the most frequent malignant primary bone tumor characterized by a high potency to form lung metastases. In this study, the effect of three oversulfated low molecular weight marine bacterial exopolysaccharides (OS-EPS) with different molecular weights (4, 8 and 15 kDa) were first evaluated in vitro on human and murine osteosarcoma cell lines. Different biological activities were studied: cell proliferation, cell adhesion and migration, matrix metalloproteinase expression. This in vitro study showed that only the OS-EPS 15 kDa derivative could inhibit the invasiveness of osteosarcoma cells with an inhibition rate close to 90%. Moreover, this derivative was potent to inhibit both migration and invasiveness of osteosarcoma cell lines; had no... |
| Tipo: Text |
Palavras-chave: Exopolysaccharides; Glycosaminoglycan; Heparin-like; Derivatives; Sulfation; Bone metabolism; Bone remodeling; Lung mestatases; Osteosarcoma. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00333/44442/44112.pdf |
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| Colliec-jouault, Sylvia; Chevolot, Lionel; Helley, Dominique; Ratiskol, Jacqueline; Bros, Andrée; Sinquin, Corinne; Roger, Olivier; Fischer, Anne-marie. |
| A new low-molecular-weight 'heparin-like' component was obtained from an exopolysaccharide produced by a mesophilic strain found in deep-sea hydrothermal vents. Data concerning the structure of the native high-molecular-weight exopolysaccharide (10(6) g/mol, 10% sulfate content) are reported for the first time. Two depolymerization processes were used to obtain low-molecular-weight (24-35 x 10(3) g/mol) oversulfated fractions (sulfate content 20 or 40%), Nuclear magnetic resonance studies indicated that after sulfation (40%), the low-molecular-weight fraction obtained by free radical depolymerization was less sulfated in the 6-O-position than the fraction depolymerized by acid hydrolysis. The free radical depolymerized product also had sulfated residues in... |
| Tipo: Text |
Palavras-chave: Sulfation; Sulfated polysaccharide; Serpin; Heparin like; Depolymerization; Anticoagulant activity; Affinity co electrophoresis. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/publication-2111.pdf |
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